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rapid excision kit  (Agilent technologies)


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  • 90

    Structured Review

    Agilent technologies rapid excision kit
    Rapid Excision Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapid+excision+kit/pmc05899665-107-50-53
    Average 90 stars, based on 1 article reviews
    rapid excision kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Antibodies to heparin-binding growth factor (HBGF) polypeptides
    Article Snippet: .. The probe was used to screen 106 plaques, two of which showed reproducible hybridization and were isolated using a Rapid Excision Kit (Stratagene). ..

    Isolation:

    Article Title: Antibodies to heparin-binding growth factor (HBGF) polypeptides
    Article Snippet: .. The probe was used to screen 106 plaques, two of which showed reproducible hybridization and were isolated using a Rapid Excision Kit (Stratagene). ..

    Sequencing:

    Article Title: Molecular Cloning of an Immunogenic Protein of Baylisascaris procyonis and Expression in Escherichia coli for Use in Developing Improved Serodiagnostic Assays
    Article Snippet: .. The single-stranded pBluescript phagemid within the lambda phage carrying the B. procyonis rag1 cDNA insert was rescued using a rapid excision kit (Stratagene) and sequenced to determine the nucleotide sequence of the rag1 cDNA insert. .. The 5′ region of this partial rag1 cDNA was identified by performing 5′ RNA ligase-mediated rapid amplification of the cDNA ends (5′ RLM-RACE) using a FirstChoice RLM RACE kit (Ambion Inc., Austin, TX).

    Article Title: C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis
    Article Snippet: Band intensities were quantified with ImageJ, and Myc-Neurog2 protein levels were normalized to β-actin. .. A 258 bp fragment corresponding to Xenopus laevis c8orf46 ( sbt1 ; ( Logan et al., 2005 )) was labeled with 32 P-dCTP using Klenow enzyme and used to screen a stage 28-30 Xenopus head cDNA library (gift of R. Harland). cDNA clones were recovered from positive phage using the Rapid Excision Kit (Stratagene) and sequenced (University of Utah Sequencing Core Facility). ..

    Clone Assay:

    Article Title: Molecular cloning and characterization of O-methyltransferases from the flower buds of Iris hollandica.
    Article Snippet: ee front matter & 2007 jplph.2006.12.002 s: COMT, caffeic acid hinidin 3-(p-coumaroyl) 5G, delphinidin 3-rham ing author.. Tel./fax: +8 ess: yabuya@cc.miyaza Summary In plants, O-methyltransferases (OMTs) play an important role in methylation of secondary metabolites, especially flavonoids and other phenylpropanoids, and two cDNA clones, IhOMT1 and IhOMT2 (Iris hollandica OMT), encoding OMTs were successfully isolated from a cDNA library of flower buds of I. hollandica.. IhOMT1 encodes an open reading frame (ORF) of 365 amino acids with calculated molecular mass of 40,193 Da and isoelectric point (pI) of 5.54, while IhOMT2, which shares 31.5% amino acid sequence identity with IhOMT1, encodes 369 amino acids with calculated molecular mass of 40,385 Da and pI of 5.50.

    Article Title: C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis
    Article Snippet: Band intensities were quantified with ImageJ, and Myc-Neurog2 protein levels were normalized to β-actin. .. A 258 bp fragment corresponding to Xenopus laevis c8orf46 ( sbt1 ; ( Logan et al., 2005 )) was labeled with 32 P-dCTP using Klenow enzyme and used to screen a stage 28-30 Xenopus head cDNA library (gift of R. Harland). cDNA clones were recovered from positive phage using the Rapid Excision Kit (Stratagene) and sequenced (University of Utah Sequencing Core Facility). ..

    In Vivo:

    Article Title: Molecular cloning and characterization of O-methyltransferases from the flower buds of Iris hollandica.
    Article Snippet: ee front matter & 2007 jplph.2006.12.002 s: COMT, caffeic acid hinidin 3-(p-coumaroyl) 5G, delphinidin 3-rham ing author.. Tel./fax: +8 ess: yabuya@cc.miyaza Summary In plants, O-methyltransferases (OMTs) play an important role in methylation of secondary metabolites, especially flavonoids and other phenylpropanoids, and two cDNA clones, IhOMT1 and IhOMT2 (Iris hollandica OMT), encoding OMTs were successfully isolated from a cDNA library of flower buds of I. hollandica.. IhOMT1 encodes an open reading frame (ORF) of 365 amino acids with calculated molecular mass of 40,193 Da and isoelectric point (pI) of 5.54, while IhOMT2, which shares 31.5% amino acid sequence identity with IhOMT1, encodes 369 amino acids with calculated molecular mass of 40,385 Da and pI of 5.50.

    Article Title: Specific induction of TaAAPT1, an ER- and Golgi-localized ECPT-type aminoalcoholphosphotransferase, results in preferential accumulation of the phosphatidylethanolamine membrane phospholipid during cold acclimation in wheat.
    Article Snippet: Cold acclimation requires substantial alteration in membrane property.. In contrast to well-documented fatty acid unsaturation during cold acclimation, changes in phospholipid biosynthesis during cold acclimation are less understood.. Here, we isolated and characterized two aminoalcoholphosphotransferase (AAPT) cDNAs, TaAAPT1 and TaAAPT2, from wheat.

    Labeling:

    Article Title: C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis
    Article Snippet: Band intensities were quantified with ImageJ, and Myc-Neurog2 protein levels were normalized to β-actin. .. A 258 bp fragment corresponding to Xenopus laevis c8orf46 ( sbt1 ; ( Logan et al., 2005 )) was labeled with 32 P-dCTP using Klenow enzyme and used to screen a stage 28-30 Xenopus head cDNA library (gift of R. Harland). cDNA clones were recovered from positive phage using the Rapid Excision Kit (Stratagene) and sequenced (University of Utah Sequencing Core Facility). ..

    cDNA Library Assay:

    Article Title: C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis
    Article Snippet: Band intensities were quantified with ImageJ, and Myc-Neurog2 protein levels were normalized to β-actin. .. A 258 bp fragment corresponding to Xenopus laevis c8orf46 ( sbt1 ; ( Logan et al., 2005 )) was labeled with 32 P-dCTP using Klenow enzyme and used to screen a stage 28-30 Xenopus head cDNA library (gift of R. Harland). cDNA clones were recovered from positive phage using the Rapid Excision Kit (Stratagene) and sequenced (University of Utah Sequencing Core Facility). ..

    Plasmid Preparation:

    Article Title: Gibberellin 2-oxidase
    Article Snippet: .. Plasmid rescue was performed using Stratagene's Rapid Excision kit. ..



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